Olive oil, especially extra virgin oilive oil (EVOO), is highly valued for its organoleptic and nutritional qualities. In this study, more than 200 monovarietal (Koroneiki) EVOO samples were collected from the main Greek olive oil producing regions. They were analysed using Flow Injection Analysis-Magnetic Resonance Mass Spectrometry (FIA-MRMS), which directly injects the oil into the mass spectrometer, to determine a metabolite profile. In parallel, the same oils were analysed using an LC-Orbitrap MS (Liquid Chromatograpy-Mass Spectrometry) platform to verify the efficiency of the method, as well as a tool to increase the identification confidence of the proposed markers.  The results obtained by FIA-MRMS analysis generated improved projection and prediction models in comparison to those of the more established LC-MS methodology. Also with FIA-MRMS, more statistically significant compounds and chemical classes were identified as quality and authenticity markers, which were associated with specific authenticity issues, i.e. geographical region, cultivation practice, and production procedures. 

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