venison authentication - News - FoodAuthenticity2024-03-29T15:58:37Zhttps://www.foodauthenticity.global/blog/feed/tag/venison%2BauthenticationA Cheaper and Simpler PCR and Lateral Flow Immunoassay Authentication of Venisonhttps://www.foodauthenticity.global/blog/a-cheaper-and-simpler-pcr-and-lateral-flow-immunoassay-authentica2021-01-10T15:56:31.000Z2021-01-10T15:56:31.000ZMark Woolfehttps://www.foodauthenticity.global/members/MarkWoolfe<div><p><a href="http://storage.ning.com/topology/rest/1.0/file/get/8409009672?profile=RESIZE_584x" target="_blank"><img class="align-center" src="http://storage.ning.com/topology/rest/1.0/file/get/8409009672?profile=RESIZE_400x" width="350" alt="8409009672?profile=RESIZE_400x" /></a></p>
<p>Venison is high value meat. Chinese researchers have developed an innovative assay which combines PCR (polymerase chain reaction) amplification of venison DNA with a lateral flow immunoassay (LFI) to visualise the PCR product. The PCR amplification used a 277 bp fragment of a venison mitochondrial DNA D-loop region, and the PCR products labelled with fluorescein isothiocyanate (FITC) and biotin were examined using a paper-based LFI strip within 5 min. The assay gave a high specificity for venison with no cross-reactivity to 17 animal and 2 plant species, and enabled the detection of raw, oven-heated, and fried venison in binary mixtures with a limit of detection (LOD) of 0.01% (w/w), In addition, the PCR-LFI test was applied to 15 commercial venison products, and the results were validated by PCR agarose gel electrophoresis.</p>
<p>Read the full paper <a href="http://pubs.acs.org/doi/full/10.1021/acsfoodscitech.0c00085" target="_blank">here</a></p></div>