pcr-lfi (2)


Venison is  high value meat. Chinese researchers have developed an innovative assay which combines PCR (polymerase chain reaction) amplification of venison DNA with a lateral flow immunoassay (LFI) to visualise the PCR product. The PCR amplification used a 277 bp fragment of a venison mitochondrial DNA D-loop region, and the PCR products labelled with fluorescein isothiocyanate (FITC) and biotin were examined using a paper-based LFI strip within 5 min. The assay gave a high specificity for venison with no cross-reactivity to 17 animal and 2 plant species, and enabled the detection of raw, oven-heated, and fried venison in binary mixtures with a limit of detection (LOD) of 0.01% (w/w), In addition, the PCR-LFI test was applied to 15 commercial venison products, and the results were validated by PCR agarose gel electrophoresis.

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Researchers in China and Canada have developed a  a highly specific and robust method to identify donkey meat that coupled a polymerase chain reaction (PCR) with lateral flow immunoassay (LFI). Donkey-specific PCR primers were designed by targeting the mitochondrial D-loop gene, and their specificity was verified in silico and in vitro against 22 species involved in meat authentication. The PCR-LFI assay gave a limit of detection as low as 0.001% w/w (raw) and 0.01% w/w (cooked) donkey meat in beef. The LFI strip-based visualisation of PCR products allowed for a 10-fold higher sensitivity than conventional gel electrophoresis, and significantly reduced the analysis time for the post-PCR analysis. This PCR-LFI is highly suitable for rapid identification of donkey or incorporation into multiplex screening protocol for other meat authentication. 

3559333008?profile=RESIZE_710x Read the full RSC research paper

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