Prawns and shrimps are high value seafood vulnerable to species substitution. Indian reseachers have developed uniplex PCR assays using species specific primers based on the mitochondrial 16S RNA and Internal Transcriber Spacer (ITS1) genes for 7 commercially important prawns and shrimps (Penaeus vannamei, Fenneopenaeus indicus, Penaeus monodon, Macrobrachium rosenbergii, Metapenaeus affinis, Heterocarpus gibbosus,and Penaeus semisulcatus). The specificity of the primers was confirmed using targeted prawn and shrimp species and untargeted fish species. The developed assays were tested using 68 purchased prawn and shrimp products, and found that 16% of the samples were mislabelled.
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