Seafood species substitution is one of the most common types of fraud.This study aims to develop new assays based on DNA to identify fresh mackerel (Scomber spp.) and mackerel species in canned products. Primers were designed to identify a DNA mini-barcoding region suitable for species identification of 4 commercial mackerel species Scomber scombrus,Scomber japonicus,Scomber colias, and scomber australaticus in processed products. Also a new assay based on RPA coupled with the lateral flow visualisation was developed for the identification of the most expensive species of fresh mackerel (Scomber scombrus).
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