ccn-1 - News - FoodAuthenticity2024-03-29T00:25:30Zhttps://www.foodauthenticity.global/blog/feed/tag/ccn-1In Vitro CRISPR-Cpf1 Assay for Differentiation between Fine and Bulk Cocoa Beanshttps://www.foodauthenticity.global/blog/in-vitro-crispr-cpf1-assay-for-differentiation-between-fine-and-b2022-07-11T16:55:38.000Z2022-07-11T16:55:38.000ZMark Woolfehttps://www.foodauthenticity.global/members/MarkWoolfe<div><p><a href="{{#staticFileLink}}10638574900,original{{/staticFileLink}}"><img class="align-center" src="{{#staticFileLink}}10638574900,RESIZE_400x{{/staticFileLink}}" width="400" alt="10638574900?profile=RESIZE_400x" /></a></p>
<p>In Ecuador, the cocoa bean clone CCN-51 is grown for bulk cocoa bean use, whereas the "Arriba Nacional" variety is highly valued for its fine cocoa flavour. An assay based on in-vitro use of CRISPR-Cpf1, which is suitable for AT-rich targets, such as the chloroplast genome (cpGenome), was developed to give a rapid differentiation between the two cocoa varieties. Two assay systems were used to digest both Arriba and CCN-51. The results were tested qualitatively by agarose gel electrophoresis, and quantitatively by capillary gel electrophoresis. Using this assay, admixtures containing 5% CCN-51 (<em>P</em> < 0.01) in Arriba and 10% Arriba (<em>P</em> < 0.05) in CCN-51were reliably detected. The application to processed cocoa products was also successful.</p>
<p>Read the abstract <a href="http://pubs.acs.org/doi/10.1021/acs.jafc.2c02537?goto=supporting-info" target="_blank">here</a></p></div>