dna based techniques (1)


This review covers the myriad of analytical techniques that can be used for honey authenticity testing. It includes various detection methods like ISCIRA, NMR, AT-FTIR, Sensors, PCR based assay united with an appropriate multivariate approach. The botanical origin authentication of honey can be determined with the application of δ13C-EA-IRMS and δ13C-LC-IRMS coupled SVM, which discriminate samples based on specific markers. LIBS, NMR, HPTLC, UHPLC, GC, and real-time PCR, can generate data that is then processed with LDA, OPLS, PCA, ANN, CNN or other chemometric tequniques. The generated data discriminate adulterated honey from pure, and it is reported that NMR coupled with PCA can detect 1% of adulterants in honey. The review concludes that there is a long way to go in this field to develop a universal technology for honey authentication and adulteration detection.

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