dna markers (2)


Rice is one othe most consumed foods globally, and different subgroups of rice varieties have different sales values e.g. Basmati rice is much more expensive than non-Basmati long grained rice. This paper by Portuguese researchers reviews DNA-based methods because they are considered particularly reliable and stable for discrimination of rice varieties. The review covers the diversity of strategies and ongoing improvements already tested, highlighting important advantages and disadvantages in terms of costs, reliability, labour-effort and potential scalability for routine fraud detection.

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In some countries, honey from native, non-domesticated species, such as Asian Apis dorsata and Apis cerana commands a much higher price than honey from the colonies of the domesticated honeybee Apis mellifera, and therefore is more vulnerable to fraud. Slovenian researchers have developed DNA  markers from a single copy ANT (adenine nucleotide translocase) gene using exon-primed intron-crossing (EPIC) primers and a double restriction protocol to obtain sequence information, which can identify the three bee species in honey. The method was developed using small extracts from 25 honeybee tissue samples and 21 honeybee products, and can be used for other bee products such as royal jelly.

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