hexaplex pcr (1)

10401197301?profile=RESIZE_400x

This study aimed to construct a two-tube hexaplex PCR (polymerase chain reaction) assay to authenticate twelve meat species. Primers were specifically designed to identify the target 12 species - horse, pigeon, camel, rabbit, ostrich, beef, as well as turkey, dog, chicken, duck, cat, and goose, and no untargeted primers were used. The assay was found to be specific, sensitive and reproducible. The assay was also tested on DNA extracted from meat which had been boiled or  microwave-cooked, and was still able to identify the meat species. The proof of principle to  identify many species simultaneously has been shown in this study, and could be adapted to cover different or less/more species.

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