isotope labelled peptides (1)

10479696676?profile=RESIZE_400xIn this paper, a quantitative method based on LC-HRMS (liquid chromatography-high resolution mass spectrometry) for the simultaneous detection and differentiation of milk type from eight different animal species (namely: cow, water buffalo, wild yak, goat, sheep, donkey, horse, and camel) was developed by detecting species specific peptides originating from casein. The use of stable isotope labelled peptides was adopted in the developed method in order to increase its accuracy and precision. The developed method was validated in-house in terms of sensitivity, accuracy, and precision. It was also used in a market survey of 46 commercial minor species’ milk products, in which 15 samples were deemed to be mislabelled.

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