Metabolic markers are considered as a promising choice for food authentication, but few metabolic markers were available to develop robust analytical methods for food authentication in routine control. Untargeted metabolomics by liquid chromatography-mass spectrometry (LC-MS) is increasingly used to discover new metabolic markers. This review summarises the general workflow, recent applications, advantages, limitations, and future needs of untargeted metabolomics by LC-MS for identifying metabolic markers. It concludes that untargeted metabolomics by LC-MS shows great efficiency to discover the metabolic markers for the authenticity assessment of biological identity, geographical origin, agricultural production, processing technology, freshness, and cause of animals’ death.
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