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There has been neither analytical methods nor specific parameters to  define milk freshness, which is an important consumer and quality issue. This study identified 8 marker molecules as indicators of milk aging, using liquid chromatography–high-resolution mass spectrometry (LC-HRMS) followed by chemometric analysis. Thirty high-quality pasteurised liquid milk samples were collected directly from a production site over a 6 week period and analysed immediately, and after storage at 2 to 8°C for 7 days to determine the markers and establish the model. The markers were then validated by challenging the model with a set of 10 milk samples, not previously analysed, and were able to clearly distinguish between the fresh pasteurised milk samples (0 days) and the stored samples (7 days). 

You can read the full paper before the 16 November 2021, and the abstract after this date.

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