Venison is high value meat. Chinese researchers have developed an innovative assay which combines PCR (polymerase chain reaction) amplification of venison DNA with a lateral flow immunoassay (LFI) to visualise the PCR product. The PCR amplification used a 277 bp fragment of a venison mitochondrial DNA D-loop region, and the PCR products labelled with fluorescein isothiocyanate (FITC) and biotin were examined using a paper-based LFI strip within 5 min. The assay gave a high specificity for venison with no cross-reactivity to 17 animal and 2 plant species, and enabled the detection of raw, oven-heated, and fried venison in binary mixtures with a limit of detection (LOD) of 0.01% (w/w), In addition, the PCR-LFI test was applied to 15 commercial venison products, and the results were validated by PCR agarose gel electrophoresis.
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