The tabs below include lists of test methods, or links, to assist location of test methods for food authenticity.
The following list includes Standard Operating Proceures prepared in the course of FSA and DEFRA Food Authenticity projects. The list is currently for information only; links or available documents will be added as available. In the mean time, SOPs or other procedures can be found by searching for project number or SOP title on the FSA or DEFRA websites.
Note: Appearance on this list does not constitute approval for a particular purpose and no warranty is given relating to the performance in practice of the SOPs listed here.
STANDARD OPERATING PROCEDURES (SOPs) PREPARED UNDER THE FOOD AUTHENTICITY PROGRAMME.
| SOP Related to Project Number | Title | Author (Organisation) | Web Link/Document |
|---|---|---|---|
| Q01055 | SOP for the Extraction of Desmin from Meat Samples | Cheryl Wells (NTU) 2006 | SOP: Extraction of Desmin |
| Q01055 | SOP for the Analysis of Desmin Enriched Meat Extracts | Cheryl Wells (NTU) 2006 | SOP: Analysis of Desmin |
| Q01066 | SOP for the Determination of the Geographical Origin of Uncooked Beef Meat.(Determination of d13C‰ and d15 N‰) | Simon Kelly (IFR) 2006 | http://sciencesearch.defra.gov.uk/... |
| Q01081 | SOP for the Determination of Corn-Fed Status of Chicken Meat using the 13C/12C Isotope Ratio of the Protein Fraction of the Meat | Paul Reece (Fera) 2008 | SOP: Corn fed chicken |
| Q01083 | SOP for the Quality Control for the Analysis of Exotic meat Samples Using Real Time PCR (TaqmanTM). | Hez Hird (Fera ) 2005 Appendix 4.1 | http://sciencesearch.defra.gov.uk/... |
| Q01083 | SOP for the Preparation of Reagents Required for RT-PCR Qualitative Analysis of Exotic Meat Species in Meat Products (CTAB) | 2005 Appendix 4.2 | http://sciencesearch.defra.gov.uk/... |
| Q01083 | SOP for the CTAB Extraction of DNA from Meat Products for Qualitative Exotic Meat Species Identification. | 2005 Appendix 4.3 | http://sciencesearch.defra.gov.uk/... |
| Q01083 | SOP for the Analysis of PCR Inhibitors in Meat Samples Using Real Time PCR (TaqmanTM) | 2005 Appendix 4.4 | http://sciencesearch.defra.gov.uk/... |
| Q01083 | SOP for the Analysis of Exotic Meat Species ( Using Real Time PCR (TaqmanTM) | 2005 Appendix 4.5 | http://sciencesearch.defra.gov.uk/... |
| Q01084 | SOP for the Quality Control and Data Analysis of the Qualitative Analysis of Commercial Meat Species in Meat Products Using Real Time PCR (TaqmanTM). | Hez Hird (Fera ) 2005 Appendix 4.1 | http://webarchive.nationalarchives.gov.uk/... |
| Q01084 | SOP for the Preparation of Reagents for the Analysis of Commercial Meat Species in Meat Samples Using Real Time PCR (TaqmanTM) (CTAB). | 2005 Appendix 4.2 | http://webarchive.nationalarchives.gov.uk/... |
| Q01084 | SOP for the CTAB Extraction of DNA from Meat Samples for Real Time PCR Identification of Commercial Meat Species (TaqmanTM) | 2005 Appendix 4.3 | http://webarchive.nationalarchives.gov.uk/... |
| Q01084 | SOP for an Internal Positive Control Protocol for the Assessment of Inhibition of the Polymerase Chain Reaction (PCR)- Performed Using ‘Real Time’ PCR and an ABI 7700 Instrument. | 2005 Appendix 4.4 | http://webarchive.nationalarchives.gov.uk/... |
| Q01084 | SOP for the Analysis of Commercial Meat Species (Pork, Beef, Lamb, Chicken and Turkey) Using Real Time PCR (TaqmanTM) | 2005 Appendix 4.5 | http://webarchive.nationalarchives.gov.uk/... |
| Q01086 | SOP for the Determination of the Geographical Origin of Poultry | Simon Kelly (IFR) 2008 | http://webarchive.nationalarchives.gov.uk/... |
| Q01093 | SOP for the Detection of Bovine and Porcine Fibrinopeptides in Raw and Cooked Meats | Paul Reece (Fera) 2007 | SOP: Fibrinogen |
| Q01095 | SOP for the Extraction of DNA from Vegetable Oil Samples | John Dooley (Campden BRI) 2006 | http://sciencesearch.defra.gov.uk/... |
| Q01095 | SOP for the Use of a DNA-Based Approach for the Detection of Unrefined Hazelnut Oil in Virgin Olive Oil. | John Dooley (Campden BRI) 2006 | http://sciencesearch.defra.gov.uk/... |
| Q01099 | SOP for the Discrimination of Salmon Species in Canned Products by PCR-RFLP Analysis using the Agilent 2100 Bioanalyser | Steve Garrett (Campden BRI) 2007 | http://sciencesearch.defra.gov.uk/... |
| Q01099 | SOP for the Discrimination of Tuna Species in Canned Products by PCR-RFLP Analysis on the Agilent 2100 Bioanalyser | Steve Garrett(Campden BRI) 2007 | http://sciencesearch.defra.gov.uk/... |
| Q01099 | SOP for Discrimination of King & Queen Scallop Species by PCR-RFLP Analysis using the Agilent 2100 Bioanalyser | Steve Garrett (Campden BRI) 2007 | http://sciencesearch.defra.gov.uk/... |
| Q01101 | SOP for a Microscopy Protocol for Identifying Mechanically Separated Meat (MSM) for Pork, Chicken and Turkey | Kathy Groves (LFR) 2007 Appendix II | http://sciencesearch.defra.gov.uk/... |
| Q01105 | SOP for the Extraction of Meat Products (Raw and Processed) and the Analysis of Added Offal. | Ellen Billett (NTU) 2012 | http://sciencesearch.defra.gov.uk/... |
| Q01106 | SOP for the Detection and Semi-Quantification of T. aestivum Addition in T. durum Pastas and Semolinas by PCR followed by Analysis using the Agilent 2100 Bioanalyser | Gordon Wiseman (Premier Foods) 2007 Appendix 8.1 | http://randd.defra.gov.uk/... |
| Q01107 | SOP for the Genetic Identification of Common and Exotic Meat Species using the Agilent 2100 Bionalyser Capillary Electropheresis System with Tepnel Bead Extraction | Hez Hird (Fera ) 2009 | http://sciencesearch.defra.gov.uk/... |
| Q01107 | SOP for the Genetic Identification of Common and Exotic Meat Species using the Agilent 2100 Bioanalyser Capillary Electropheresis System with CTAB Extraction | Hez Hird (Fera) 2009 | http://sciencesearch.defra.gov.uk/... |
| Q01108 | SOP for the Quantitative Analysis of Adulteration of Basmati Rice with the Varieties - Sherbati, Mugad Sugandha, PAK 386 or Superfine using the Agilent 2100 Bioanalyser (SOP2-RM201) | Katherine Steele (Food DNA Services 2008 | FSA SOP: Basmati Quant Bioanalyser RM201 |
| Q01108 | SOP for the Quantitative Analysis of Adulteration of Basmati Rice with the Varieties - Sherbati, Mugad Sugandha, PAK 386 or Superfine using the Agilent 2100 Bioanalyser (SOP3-INDEL B8) | Katherine Steele (Food DNA Services) 2008 | FSA SOP: Quant Indel Bioanalyser |
| Q01108 | SOP for the Detection of Non-Permitted Rice Varieties by INDEL-PCR Analysis using the Agilent 2100 Bioanalyser (SOP1) | Katherine Steele (Food DNA Services) 2008 | FSA SOP: Qual Indel Bioanalyser |
| Q01108 | SOP for the Adulteration of Basmati Rice with Sherbati, Mugad Sugandha, Pak 386 or Superfine (Beckman Quantitative SSR) | Katherine Steele (Food DNA Services) 2008 | FSA: Beckman Quant (not in FSA SOP format) |
| Q01108 | SOP for the Identification of Selected Basmati Rice Varieties (Beckman qualitative SSR) | Katherine Steele (Food DNA Services) 2005 | FSA: Basmati Beckman Qual (not in FSA SOP format) |
| Q01109 | SOP for the Genetic Identification of Common African Wildmeat Species using the Agilent 2100 Bioanalyser | Rob Ogden (Food DNA Services) 2009 | FSA SOP: Bushmeat Agilent |
| Q01114 | SOP for the Determination of Grapefruit Juice in Orange Juice using the Agilent 2100 Bioanalyser | Angus Knight (LFR) | |
| Q01114 | SOP for the determination of Mandarin Juice in Orange Juice using the Agilent 2100 Bioanalyser | Angus Knight (LFR) 2010 | FSA: Mandarin Bioanalyser |
| Q01118 | SOP for the Detection and Speciation of Gelatine in Vegetarian Meals | Helen Grundy (Fera) Appendix 1 2008 | http://sciencesearch.defra.gov.uk/... |
| Q01122 | SOP for the Determination of Theobromine and Caffeine in Cocoa and Chocolate by HPLC | Alan Richards (Durham PA) 2011 Appendix 7 | http://sciencesearch.defra.gov.uk/... |
| Q01123 | SOP for the Determination of the ‘Global’ Deuterium/Hydrogen (2H/1H) and Oxygen-18/Oxygen-16 (18O/16O ) Ratio of Bulk Materials. | Simon Kelly (IFR) 2008 | http://sciencesearch.defra.gov.uk/... |
| Q01123 | SOP for the Determination of the ‘Global’ or ‘Average’ Carbon-13/Carbon-12 (13C/12C) and Nitrogen-15/Nitrogen-14 (15N/14N) Ratio of Bulk Materials. | Simon Kelly (IFR) 2008 | http://sciencesearch.defra.gov.uk/... |
| Q01123 | SOP for the Determination of the ‘Global’ or ‘Average’ Sulphur-34/Sulphur-32 (34S/32S) Ratio of Bulk Materials | Simon Kelly (IFR) 2008 | http://sciencesearch.defra.gov.uk/... |
| Q01123 | SOP for Strontium-87 Isotope Analysis of Organic Material | Simon Kelly (IFR) 2008 | http://sciencesearch.defra.gov.uk/... |
| Q01123 | SOP for Trace Element Analysis of Organic Material | Simon Kelly (IFR) 2008 | http://sciencesearch.defra.gov.uk/... |
| Q01129 | The Development and Validation of DNA Marker Methods for the Verification of Meat from Wild Boar. | Hez Hird (FERA) | |
| Q01130 | SOP for the Genetic Identification of Traditional Breeds of Pig using DNA SNP Markers | Rob Ogden (Gen-Probe) 2011 (Appendix IVa) | http://sciencesearch.defra.gov.uk/... |
| Q01130 | SOP for the Genetic Identification of Traditional Breeds of Cattle using DNA SNP Markers | Rob Ogden (Gen-Probe) 2011 (Appendix IVb) | http://sciencesearch.defra.gov.uk/... |
| Q01131 | SOP for a Microscopy Protocol for Identifying Mechanically Separated Meat (MSM) for Beef and Lamb. | Kathy Groves (LFR) 2011 Appendix II | http://sciencesearch.defra.gov.uk/... |
| Q01132 | SOP for the Micro-Method for the Determination of Hydroxyproline in Gelatine Extracts | Helen Grundy (FERA) | |
| Q01132 | SOP for the Isolation of Gelatine from Chicken Fillet Preparations | Helen Grundy (FERA) | |
| Q01132 | SOP for the LC/MS/MS of Tryptic Digests of Chicken Exudate Extracts to Determine the Species Origin of Gelatine | Helen Grundy (FERA) | |
| Q01132 | SOP for the Tryptic Digestion of Gelatine | Helen Grundy (FERA) | |
| FA0106 | SOP for the Extraction of a Heart Marker Protein in Raw and Processed Meat Products and Its Subsequent Detection and Quantification by an ELISA Assay | Ellen Billet (NTU) | SOP For heart marker protein extraction |
| FA0114 | SOP for the Detection of Previously Frozen Poultrymeat by Determination of HADH Activity | LGC 2013 Appendix 1 | http://randd.defra.gov.uk/Default.asp... |
| FA0116 | Genetic Identification of Fish Species using DNA Barcoding | Labelfish consortium (2014) | FoodAuthenticity Documents site |
| FA0117 | SOP for Development of a Two-Step Method to Determine Vegetable Oil Species in Vegetable Oil Mixtures | Tassos Kaodis (University of Belfast) 2013 | http://sciencesearch.defra.gov.uk/... |
| FA0118 | SOP for the Genetic Identifications of Cod, Hake and Sole Populations using DNA SNP Markers | Rob Ogden (Trace Wildlife Forensics) 2014 Appendix III | Download from FoodAuthenticity Documents site |
| FA0122 | SOP for the extraction of offal marker proteins in raw and processed meat products, and their subsequent detection and semi-quantification | Lyndsey Durose, Nottingham Trent University, 2016 | |
| FA0125 | SOP for the Genetic Identification of Traditional Cow Breeds using DNA SNP Markers | Rob Ogden (Trace Wildlfe Forensics) 2012 | https://documents.foodauthenticity.global/... |
| FA0125 | SOP for the Genetic Identifications of Traditional Pig Breeds using DNA SNP Markers | Rob Ogden (Trace Wildlife Forensics ) 2012 | http://sciencesearch.defra.gov.uk/... |
| FA0137 | SOP for the Environmental Monitoring of Meat Processing Plants | LGC | |
| FA0146 | Draft standard operating procedure for the quantitation of horse dna relative to mammalian dna in raw meat samples | LGC, Teddington | |
| FA0158 | Development and validation of the proposed methodology to verify vegetable oil species in mixtures of oil | Queen's University, Belfast |
