In this paper (open access) the authors developed a rapid PCR-based test protocol for three species of tuna (Thunnus thynnus (Blue Fin Tuna), Thunnus albacares, and Katsuwonus pelamis) under simulated conditions for canned and flavoured products. Home-made canned simulants were prepared by mixing each fish tissue of the three tuna species with salt, pepper, paprika, onion, oil, vinegar, and tomato followed by frying and boiling. DNA was then isolated from the home-made canned products. Binary mixtures were prepared using the isolated DNA in various percentages of adulteration that ranged from 1 to 100%. DNA was extracted, followed by amplification by rapid small-scale PCR using species-specific primers. The PCR products were hybridized (10 min) to specific probes and applied to the rapid sensing device. The signal was observed visually in 10–15 min using gold nanoparticle reporters. The authors report that the method was reproducible and specific for each tuna species and 1% of tuna adulteration (in the isolated DNA) could be detected with the naked eye.
photo by Farhad Ibrahimzade on UnsplashP
Comments