Honey authentication is an area where there is a continual “arms race” between analytical methods and methods of adulteration or other fraud. This review (open access) provides a comprehensive overview of honey authenticity challenges and related analytical methods. It describes direct and indirect methods of honey adulteration and the existing challenges in current detection methods and market supervision approaches. The authors focus on the advantages, disadvantages and scopes of integrated metabolomic workflows involving sample processing procedures, instrumental analysis techniques and chemometric tools. They discuss various improved microscale extraction methods combined with hyphenated instrumental analysis techniques and chemometric data processing tools. They conclude that the future of honey authenticity determination will involve the use of simplified and portable methods, which will enable on-site rapid detection and transfer detection technologies from the laboratory to the industry.