pcr (3)

Single PCR for 14 Commercialised GM Soy Events

12941347260?profile=RESIZE_400xIn this study (purchase required), 14 widely commercialized GM soybean events, including GTS40-3-2, MON89788, CV127, A2704-12, A5547-127, 305423, 356043, MON87701, MON87705, MON87708, MON87769, SYHT0H2, FG72, and DAS-444Ø6-6 were utilised as targets for event-specific identification. A high-throughput detection method was established by integrating a single universal primer multiplex PCR (SUP-M-PCR) with capillary electrophoresis  The authors report that this method enables the identification of 14 soybean events in a single PCR reaction, addressing the limitations of conventional multiplex PCR such as self-inhibition and amplification variations between different primers. Furthermore, it significantly enhances the sensitivity and accuracy of previous multiple detection methods, achieving a detection sensitivity of 0.05% (w/w). They conclude that this approach improves detection efficiency and holds promising potential for the identification and detection of the 14 targeted GM soybean events and their derivatives.

Photo by Meredith Petrick on Unsplash

Read more…

 9928071854?profile=RESIZE_400x

Meat adulteration/substitution has long been a source of concern for a variety of reasons, including public health, religious considerations, wholesomeness, unhealthy competitiveness in the meat industry, and fraud. Therefore, a range of kits have been developed to permit rapid, precise, and specific identification of meat species. These are based on DNA methods (PCR and Real-Time PCR) and Enzyme-Linked Immunosorbent Assay (ELISA). In response to these developments, Advance Market Analytics has published a new research publication on “Global Meat Species Identification Kits Market Insights, to 2026”.

The report has 9 chapters:

Chapter 1: Introduction, market driving force product Objective of Study and Research Scope the Meat Species Identification Kits market

Chapter 2: Exclusive Summary – the basic information of the Meat Species Identification Kits Market.

Chapter 3: Displaying the Market Dynamics- Drivers, Trends and Challenges & Opportunities of the Meat Species Identification Kits

Chapter 4: Presenting the Meat Species Identification Kits Market Factor Analysis, Porters Five Forces, Supply/Value Chain, PESTEL analysis, Market Entropy, Patent/Trademark Analysis.

Chapter 5: Displaying the by Type, End User and Region/Country 2015-2020

Chapter 6: Evaluating the leading manufacturers of the Meat Species Identification Kits market which consists of its Competitive Landscape, Peer Group Analysis, BCG Matrix & Company Profile

Chapter 7: To evaluate the market by segments, by countries and by Manufacturers/Company with revenue share and sales by key countries in these various regions (2021-2026)

Chapter 8 & 9: Displaying the Appendix, Methodology and Data Source

 A free example copy of the report can be obtained.

Read more…

3822242868?profile=RESIZE_710xIndian researchers have developed a PCR method based using 3 different primers on the mitochondrial gene 12S rRNA. The method produces a cow specific amplicon (346bp) and a buffalo specific amplicon (220bp). The method can detect 0.5% addition of buffalo milk to cow milk. Although this test has been used in India to determine buffalo milk adulteration of cow milk, it could be adapted to determine cow milk adulteration of buffalo milk e.g. in the case of mozzarella cheese.

Read the full research paper

Read more…