CRISPR (clustered regularly interspaced short palindromic repeats)-Cas(CRISPR-associated protein) is derived from a microbial antiviral system and is commonly associated with cutting edge gene editing. However, the system can also be adapted for diagnostic applications inclusive of novel approaches for food authenticity and adulteration testing.
In this paper, the Chinese research team coupled PCR amplification with a CRISPR-Cas12a based colorimetric system to indicate the presence of DNA barcoding targets by a visible colour change. The whole detection process including PCR amplification and TMB ( 3,3′,5,5′-tetramethylbenzidine sulfate) colorimetric analysis can be completed within 90 minutes.This sensitive assay was verified by the identification of four important species of pufferfish,
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