This review article (purchase required) covers recent developments in loop-mediated isothermal amplification (LAMP) devices, particularly when coupled with microfluidic chips, for applications as point-of-use tests for meat and seafood species authentication.
While PCR-based methods remain the gold standard for assessment of the species authenticity, the authors consider that there is an urgent need for alternative testing platforms that are rapid, accurate, simple, and portable. Owing to its ease of use, low cost, and rapidity, LAMP is becoming increasingly used method in food analysis. The authors outline how the features of LAMP have been leveraged for species authentication testing of meat and seafood products. LAMP detection is simple and rapid. To make it truly instrument-free it needs an end-point visual detection, and so the authors review the principles of various end-point colorimetry methods. They also summarise different strategies to either suppress the nonspecific amplification or to avoid the results of nonspecific amplification.
Comments