lc-ms (3)


Olive oil, especially extra virgin oilive oil (EVOO), is highly valued for its organoleptic and nutritional qualities. In this study, more than 200 monovarietal (Koroneiki) EVOO samples were collected from the main Greek olive oil producing regions. They were analysed using Flow Injection Analysis-Magnetic Resonance Mass Spectrometry (FIA-MRMS), which directly injects the oil into the mass spectrometer, to determine a metabolite profile. In parallel, the same oils were analysed using an LC-Orbitrap MS (Liquid Chromatograpy-Mass Spectrometry) platform to verify the efficiency of the method, as well as a tool to increase the identification confidence of the proposed markers.  The results obtained by FIA-MRMS analysis generated improved projection and prediction models in comparison to those of the more established LC-MS methodology. Also with FIA-MRMS, more statistically significant compounds and chemical classes were identified as quality and authenticity markers, which were associated with specific authenticity issues, i.e. geographical region, cultivation practice, and production procedures. 

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German and Italian researchers have used a proteomics based assay to distinguish wild from farmed salmon. A total of 13 farmed and 13 wild Canadian salmon (Salmo salar) species were extracted and digested with trypsin. The peptide digest was analysed by an optimised LC- MS system(quadrupole time-of-flight mass spectrometer) followed by statistical analysis based on principal component (PC) analysis.This untargeted approach, using a data-independent acquisition MS scheme, demonstrated the ability to effectively discriminate salmon belonging to the two classes. Furthermore, selected peptides showing high loadings on PC1 could represent potential targeted candidate peptide markers able to discriminate farmed from wild-type salmon.

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Coffee is currently the second largest commodity on the world market. Brazilian researchers have written a comprehensive review on the development and use of chromatograpy from paper to gas and hplc, and finally ultra-performance liquid chromatography coupled with tandem mass spectrometry to confirm adulteration and fraud in coffee.  

 Read the full review here

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