The German Federal Office of Consumer Protection and Food Safety (BVL) has published new guidelines on verification of dPCR methods. These were developed by the international working group “Development of methods for identification of foodstuffs produced by means of genetic engineering techniques”. The guideline provides practical recommendations for transferring the real-time PCR to digital PCR and for verifying the digital PCR method. The guideline is applicable to analysis of GMO in food, feed and seed. It includes initial validation of the dPCR system (e.g. uncertainty in the reaction volume of each partition), transfer of conditions from RT-PCR to dPCR, dPCR performance criteria, and validation of duplex dPCR for transgene and reference gene quantification.
For an introductory explainer of dPCR see FAN’s analytical methods pages.
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