This paper (purchase required, US$ 38) reports the development of a point-of-use loop mediated isothermal (LAMP) assay to detect soybean adulteration in vegetable oils.
The LAMP primers of rtLAMP were designed based on the ITS2 sequence and further were used for validating the specificity and sensitivity.
The authors report that the LAMP primers of rtLAMP exhibited high specificity for amplifying soybean DNA extracted from oil samples, achieving authentication within 30 min at an isothermal temperature of 62 °C. The sensitivity of rtLAMP was 103-fold greater than that of real-time PCR (1 ng) for soybean DNA detection. rtLAMP could detect down to 10% of soybean oil adulterated in the edible oil sample. Moreover, all commercial edible oil products were positively identified (6/6) using rtLAMP, whereas the real-time PCR only identified 4 out of 6.
For an overview of LAMP principles see our Analytical Explainers page.
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