Further to the review of methods used in the EU co-ordinated survey of honey collected 2021-22 (“From the Hives” survey) – see previous blog here:
The Joint Research Centre of the European Commission have now published (open access) more details of some of the test methods used and results interpretation. This publication relates particularly to the two qualitative Liquid Chromatography–High-Resolution Mass Spectrometry (LC-HRMS) methods that were developed to detect mannose (Man), difructose anhydride III (DFA III), 2-acetylfuran-3-glucopyranoside (AFGP), and oligo-/polysaccharides with degrees of polymerization (DPs) of 6 to 11.
The presence of mannose and unusual oligo-/polysaccharides was the main reason that many of the samples were flagged as “suspicious” in the previously published report.
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