In 2009, the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (dMIQE) guidelines established standards for the design, execution, and reporting of quantitative PCR (qPCR) in research.
The expansion of qPCR into numerous new domains has driven the development of new reagents, methods, consumables, and instruments, requiring revisions to best practices that are tailored to the evolving complexities of contemporary qPCR applications.
Building on the collaborative efforts of an international team of researchers, updates, simplifications, and new recommendations to the original MIQE guidelines are presented, designed to maintain their relevance and applicability in the context of emerging technologies and evolving qPCR applications.
MIQE 2.0 has been added to FAN's Quality Section. Read MIQE 2.0 (DOI: https://doi.org/10.1093/clinchem/hvaf043).
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