In this paper (open access, Chinese language) the authors report a quick method for detecting the authenticity of meat products based on multienzyme isothermal rapid amplification (MIRA) coupled with a lateral flow dipstick (LFD).
Pig, cattle, sheep, chicken, and duck-specific mitochondrial gene sequences were selected through GenBank, and intra-species conserved and inter-species specific target fragments were compared using the DNAStar Megalign software. According to the design principles of MIRA primers and probes, target gene-specific primer and probe sets were designed and screened systematically through positive, negative and blank controls to determine the species-specific primers and probes. The reaction system, temperature, and time were optimized.
The authors reported that the method performed well when assessed as a rapid point-of-use screening method. The average false positive rate of this method was 3.49%, and the false negative rate was 3.54%, which meets the requirements of on-site rapid testing.
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