This study (open access) advances the field of “natural” vanilla flavour authentication, and of geographic origin of vanilla pods, by investigating minor volatile organic compounds and their isotopic ratios. Vanilla pods from the two main vanilla species, V. planifolia and V. tahitensis, were investigated using GC-MS/MS to analyze their aromatic profile and GC-C/Py-IRMS to determine compound-specific isotope ratios, providing, for the first time, detailed and authentic isotopic and aromatic profiles.
The researchers quantified more than 50 volatile compounds in different vanilla pods.
A key finding was the confirmation—through UHPLC-HRMS analysis—that ethyl vanillin and its glucoside precursors were absent in genuine vanilla extracts, reinforcing that the detection of ethyl vanillin remains a reliable marker of fraud.
The authors conclude that their study provides new insights into the natural pathways of biosynthesis in vanilla. For the first time, compound-specific isotope analysis has been applied to minor aromatic compounds in vanilla pods, opening new avenues for their use in authentication and botanical and geographical traceability of vanilla flavours. The study pioneers the application of isotope analysis to authentic vanilla extracts spiked with synthetic ethyl vanillin, enabling a more precise assessment of the impact on the isotopic composition of foods flavoured with natural vanillin following fraudulent augmentation with small amounts of ethyl vanillin.
Photo by Jocelyn Morales on Unsplash
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