High performance liquid chromatography and high-resolution mass spectrometry (HPLC-MS/MS) was used to identify gelatin from seven commercial cyprinid fishes;, black carp, grass carp, silver carp, bighead carp, common carp, crucian carp, and Wuchang bream.
By comparison with theoretical mammalian collagen (bovine and porcine collagen), the common and unique theoretical peptides were found in the collagen of grass carp, silver carp, and crucian carp, respectively. Seven common characteristic peptides were obtained from the fish gelatins. Moreover, 44, 36, and 42 unique characteristic peptides were detected in the gelatins of grass carp, silver carp, and crucian carp, respectively.
The researchers concluded that the combined use of common and unique characteristic peptides could verify fish gelatin in comparison with mammalian gelatin.