Extracting DNA of suitable quality and quantity from a test sample is a fundamental upstream step that underpins the confidence in a number of downstream analytical molecular biology based methods (e.g., qPCR. dPCR, NGS, etc.,).
This official guidance document provides advice on the selection and use of fit for purpose DNA extraction methods. Whilst this guidance uses the example of DNA extraction in the context of official controls for the analysis of genetically modified organisms (GMOs), the principles it describes are universally applicable to all DNA based methods including those for food authenticity.
Advice is provided on the selection of different protocols and decision support systems, and guidance provided on validation approaches and the assessment of DNA quality parameters, further illustrated with practical examples/solutions based on extensive collective experiences.
Access guidance: DOI: 10.2760/76162 (online)
This guidance has also been added to the Quality section of this website.