In this paper (£29.95 purchase required) the authors report the development of a point-of-use test for chicken adulteration (down to 1% w/w) in meat products. They report that the test takes 40 minutes with a per-test cost of around US$1.
They used a swab-based sampling protocol coupled with a dedicated HPV10 nucleic acid releaser. They report that this approach enables efficient DNA release from swab samples within a brief lysis step while minimizing subsequent amplification inhibition. This simplified sampling strategy was further integrated with loop-mediated isothermal amplification (LAMP) and naked-eye colorimetric detection, resulting in a fully integrated “swab-to-result” platform.
They report that validation using commercially available meat products confirmed consistent and reliable detection performance at 1% w/w adulteration.
Photo by Scott Eckersley on Unsplash
