This study (purchase required) builds on previously reported work that used real-time Loop Mediated Isothermal Amplification (LAMP) assays to detect a single GM target. To increase the efficiency and scope of the assay, the authors have developed a multiplex real-time LAMP simultaneously targeting Figwort Mosaic Virus promoter (P-FMV) that constructs region between the Cauliflower Mosaic Virus 35S promoter and cry1Ac gene (p35S-cry1Ac) and neomycin phosphotransferase II (nptII) marker gene. The assay could detect as low as 0.1% for each GM target within 45 minutes.
The authors believe that this configuration and application - multiplexing in real-time LAMP using the Genie II system with applicability in GM detection – is novel. They conclude that the developed method provides rapid, on-site, and real-time GM detection in seeds and food products.
For an explanation of LAMP, see FAN’s analytical method explainers on DNA techniques.
Graphical abstract from the paper.
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