isothermal amplification (2)

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In the last decades, the demand for molecular tools for authenticating and tracing agri-food products has significantly increased. Food safety and quality have gained an increased interest for consumers, producers, and retailers, therefore, the availability of analytical methods for the determination of food authenticity and the detection of major adulterations takes on a fundamental role.


Among the different molecular approaches, some techniques such as the molecular markers-based methods are well established, while some innovative approaches such as isothermal amplificationbased methods and DNA metabarcoding have only recently found application in the agri-food sector.

In this review, we provide an overview of the most widely used molecular techniques for fresh and processed agri-food authentication and traceability, showing their recent advances and applications and discussing their main advantages and limitations. The application of these techniques to agrifood traceability and authentication can contribute a great deal to the reassurance of consumers in terms of transparency and food safety and may allow producers and retailers to adequately promote their products.

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Fish species substitution is a major global fraud problem. Atlantic salmon (Salmo salar) is widely appreciated, especially smoked or as fillets, and is a high value  fish in many export markets, and thus vulnerable to substitution. Chinese researchers have developed a real-time DNA assay based on isothermal  amplification (LAMP-real time fluorescence loop-mediated isothermal amplification) of a specific section of the mitochondrial cytochrome b gene, which is sensitive, and more rapid than normal PCR thermocycling. The assay was specific to Atlantic salmon without any cross-reactivity with 11 non-targeted salmonoid species. It was tested on 30 commercial salmon products collected in local markets in Nanjing, and only six were found to contain Atlantic salmon.

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