This paper (purchase required) describes the development and validation of a highly sensitive lateral flow immunoassay (LFIA) test kit for detecting trace pork in meat products. A rapid bead-based sample extraction was developed (1–5 min) for the biomarker (porcine IgG). The authors report that total test time to result was 20 minutes. The reported detection limit was 0.001 % (w/w), which is 5–500 × more sensitive compared to current commercial lateral flow kits. The LFIA was validated with a range of meat and processed food products, confirming its high specificity to pork without cross-reactivity to other animal species or non-meat ingredients. Moreover, a long-term stability study confirmed that the LFIA maintained its performance at room temperature storage for 2 years.
