This study (purchase required) shows the potential for stable isotope ratio analysis (SIRA) to be used to verify whether the cocoa in bean-to-bar chocolate originated from the Amazon region. Higher-sugar chocolate is too complex a product to test in this way. Such tests will be important for checking compliance with future EU Deforestation Regulations. The proposed method can also be used to estimate the cocoa content of chocolate.
Chocolate is a complex product in terms of its carbon isotope distribution. Cocoa, from a C3-photosynthetic plant, is its main raw material, while sugar from sugarcane (C4-metabolism) is also commonly included. The authors analyzed the δ13C and δ15N composition of Brazilian chocolates, including Conventional, Bean-to-bar, and Imported brands across White, Milk, Semisweet, Dark <70 %, and Dark ≥70 % versions. They report that conventional White, Milk, and Semisweet chocolates showed no significant isotopic differences, with average δ13C values around −22 ‰, indicating high C4-derived ingredient content. Bean-to-bar chocolates presented δ13C values 2–3 ‰ lower, and those made with Amazon cocoa were ∼1.5 ‰ lighter than those from Atlantic Forest, enabling accurate prediction of cocoa origin. Imported chocolates showed even lighter δ13C values, suggesting greater use of C3-based ingredients. δ13C and δ15N values also enabled reliable estimation of cocoa content.
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